This package is in development for a presentation at Galaxy-Bioconductor Community Conference 2025.
The main purpose is to illustrate analysis of MCMICRO spatial proteomics experiments.
Given an image and associated protein quantifications from the platform, what computations facilitate biological interpretation?
An important determinant of statistical analysis is the approach to defining distance between cells. Here’s a default cell-cell distance ‘graph’:
Here’s an alternative with neighbor distances for a given cell produced only for the three nearest cells.
Statistics concerning spatial distributions of cell attributes depend upon the definition of cell-cell distance.
Getis-Ord’s Gi* statistic is reported on a Z-score scale and has been used to characterize hot spots in spatial surveys.
This package will provide code to help explore aspects of such data and analyses.