All functions

XenSPEP-class

manage SpatialExperiment with parquet references

XenSPEP()

XenSPEP (SpatialExperiment with parquet references) constructor

cache_mtif()

cache and/or retrieve path to an ome.tif file for demonstration

cache_sfeLung_ntx()

cache and/or retrieve path to an SFE of V1 lung demo data from 10x

cache_xen_luad()

cache and/or retrieve path to Xenium Lung Adenocarcinoma example data, zipped SPEP accompanied by parquet

cache_xen_pdmel_lite()

counts-in-memory version of melanoma 5k dataset

cache_xen_prost_lite()

counts-in-memory version of prostate 5k dataset

clip_rect()

restrict XenSPEP to cells with centroids in specified rectangle, also restrict boundary and transcript location features

demoapp()

simple app to explore an image

e2sym()

helper function to map ENS ids to symbols

e79sym

mapping from ENSG to symbols based on EnsDb.Hsapiens.v79

getCellBoundaries(<XenSPEP>)

method for cell boundary extraction

getCellBoundaries()

method for cell boundary extraction

getNucleusBoundaries(<XenSPEP>)

method for nucleus boundary extraction

getNucleusBoundaries()

method for nucleus boundary extraction

getTranscripts(<XenSPEP>)

method for transcript extraction

getTranscripts()

method for transcript extraction

ggprep_seg()

prepare a XenSPEP for ggplot2 visualization

ingest_xen()

produce a pre-loaded XenSPEP (SpatialExperiment with parquet references)

loadGeometry(<XenSPEP>)

read and bind parquet data to XenSPEP

loadGeometry()

read and bind parquet data to XenSPEP

make_cropper()

use spatialdata bounding_box_query to confine attention

plot_xen_ggprep()

plot method for ggplot2-prepared XenSPEP

print_xen_ggprep()

print method for ggplot2-prepared XenSPEP

rast_zarr()

produce an array from selected image in a spatialdata zarr store

reset_parq_paths()

utility for dealing with cached Xen_SPEP in temp folder

restoreZipXenSPEP()

use unzip, readRDS, and loadGeometry to restore a XenSPEP

show(<XenSPEP>)

display aspects of XenSPEP

`[`(<XenSPEP>,<ANY>,<ANY>,<ANY>)

formal bracket definition, that leaves parquet geometry information alone.

view_seg()

naive polygon viewer

view_seg_g2()

naive polygon viewer, will indicate presence of transcripts for two genes in cells

xdims()

helper function for XenSCE show method, producing dimensions for geometry information

zipXenSPEP()

serialize the collection of XenSPEP and parquet with zip